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Encompassing Amino Acids: 18-238
Applications: Useful for labeling PD-1 expressing cells for flow cytometry or immunohistochemistry.
Description: Human Secreted Programmed Cell Death Ligand 1 (PD-L1)-Fc fusion protein, also known as, CD47 or B7-H1, GenBank Accession No. NM_021893, a.a 18-238, fused at the C-terminus to the Fc portion of human IgG1, expressed in a HEK293 cell expression system. MW = 51 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation. The protein was randomly labeled with RPE on accessible lysine residues.
Format: Aqueous buffer solution
Formulation: 8 mM Na Phosphate pH 7.4, 110 mM NaCl, 2.2 mM KCl and 20% glycerol.
Genbank: NM_021893
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: FC Fusion, PE Labeled
Uniprot: Q9EP73
Warnings: Avoid freeze/thaw cycles. Protect from light.
Biosafety Level: Not applicable (BSL-1)
References: 1. Brahmer, S., et al., N Engl J Med. 2012 Jun;366: 2455-24652. Powels, T., et al., Nature. 2000 Oct; 515(7528):558-562.