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Encompassing Amino Acids: 20-219
Applications: Useful for the study of protein-protein interaction and in vitro and in vivo protein function.
Assay Conditions: PD-1-PD-L2 binding activity, measured using the using the PD-1[Biotinylated]:PDL2 Inhibitor Screening Assay Kit, #72006
Description: Human secreted Programmed Death Ligand 2 (PD-L2)-Fc fusion protein, also known as CD273, PDCDL1G2, and B7-DC, GenBank Accession No. NM_025239, a.a. 20-219, fused at the C-terminus to the Fc portion of human IgG1 expressed in a HEK293 cell expression system. MW = 49 kDa.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, pH 7.4, and 20% glycerol
Genbank: NM_025238
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal Fc fusion (Human IgG1)
Uniprot: Q9BQ51
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Latchman, Y., et al., Nat. Immunol. 2001 Mar;2(3):261-8.
2. Saunders, P.A., et al., Eur. J. Immunol. 2005 Dec;35(12):3561-9.