Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl
2 , 0.1 mg/ml BSA,200 µM cAMP, 2.5 kU 5'-nucleotidase, and PDE11A at 37°C for 20 min.Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate groupwhich is detected by Malachite Green Reagent.
Description: Human PDE11A (GenBank Accession No.BC112393), full length with N-terminal GST-tag, MW= 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 10 mM glutathione, and 3 mM DTT
Genbank: BC112393
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q9HCR9
Unit Definition: One unit is defined as the amount of enzymethat will convert 1 pmole of 3' , 5' -cAMP to5' -AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Loughney K, et al., Int. J. Impot. Res. 2005, 17(4):320-5.
2. Weeks JL, et al., Int. J. Impot. Res. 2005, 17(1):5-9.Application Reference(s):
1.
Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014)
2. A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013)
3. The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)
English
Deutsch
