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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE1A Assay Kit, Cat. # 60310. Assay conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE1A at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE1A, isozyme 4 (GenBank Accession No. NM_001258313.1), full length with N-terminal GST-tag, MW= 88.3 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, and 20% glycerol
Genbank: NM_001258313.1
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: P54750-4
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Nagel, D.J., et al., Circ. Res. 98 (6), 777-784 (2006).
2. Goraya, T.A., et al., J. Biol. Chem. 279 (39), 40494-40504 (2004).Application Reference(s):
1. Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A (2015)
2. Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014)
3. The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)
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