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Encompassing Amino Acids: 2-748(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: 10 mM Tris-HCl,pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA,0.05% Tween-20, 200 µM cAMP, 2.5 kU 5'-nucleotidase, andserial dilutions of PDE4D7 at 37°C for 20 min.Quantified by 5'- nucleotidase cleaving the5' -AMP product and releasing thephosphate group which is detected byMalachite Green Reagent.
Description: Human PDE4D7 (GenBank Accession No. NM_001165899), a.a. 2-748(end) with N-terminal GST-tag, MW=111 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 3 mM DTT, 20mM Glutathione and 10% glycerol.
Genbank: NM_001165899
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: Q08499
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wang, D., et al., (2003). Cell Signal. 15(9):883-91.
2. Houslay, M.D. (2005). Mol Pharmacol. (2005). 68(3):563-7
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