Products from BPS Bioscience require a minimum order value above 400€Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Assayed using BPS's PDE8A kit, Cat. # 60380. Assay conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl
2, 0.05% Tween-20, 0.1 mg/ml BSA, 200 µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of PDE8A1 at 37°C for 20 min. Quantified by 5'-nucleotidase cleaving the 5'-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent.
Description: Human PDE8A1 (GenBank Accession No. NM_002605), full length with N-terminal GST-tag, MW= 119 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110 mMNaCl, 2.2mM KCl, 0.04% Tween-20, 20%glycerol, and 3 mM DTT.
Genbank: NM_002605
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag
Uniprot: O60658
Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3', 5'-cAMP to 5'-AMP per minute at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Wu P. and Wang P. Proc. Natl. Acad. Sci. U.S.A. 101 (51), 17634-17639 (2004).
2. Gamanuma M. et al., Cell. Signal. 15 (6), 565-574 (2003).Application Reference(s):
1.
Characterization of the binding properties of T-773 as a PET radioligand for phosphodiesterase 10A (2015)
2. Genetic deletion and pharmacological inhibition of phosphodiesterase 10A protects mice from diet-induced obesity and insulin resistance (2014)
3. A yeast-based chemical screen identifies a PDE inhibitor that elevates steroidogenesis in mouse Leydig cells via PDE8 and PDE4 inhibition (2013)
4. The novel phosphodiesterase 10A inhibitor THPP-1 has antipsychotic-like effects in rat and improves cognition in rat and rhesus monkey (2013)