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Amino Acid Sequence: SLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
Applications: Useful for cell culture and for the study of signaling pathways. Also useful for receptor binding studies, screening inhibitors, and selectivity profiling.
Biological Activity: The ED50 as determined by the dose-dependent stimulation of BALB/c 3T3 cells proliferation is < 1.0 ng/ml.
Description: Human recombinant PDGF-BB (GenBank Accession No. NM_002608) is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total a.a.) expressed in Saccharomyces cerevisiae.
Endotoxin Level: < 1EU per µg of PDGF-BB as determined by the LAL method.
Format: lyophilized protein
Formulation: Lyophilized from a 0.2 µm filtered 0.02M acetic acid solution, pH 6.5.
Genbank: NM_002608
Purity: ≥98% by SDS-PAGE and HPLC analysis.
Reconstitution: Reconstitute at 0.1-1.0 mg/ml in distilled water. This solution can then be diluted into other buffers. To maximize product collection from vial surface, vortex briefly and then spin down to recollect the liquid.
Storage Stability: ≥2 years (lyophilized) at -20°C.
Tags: none
Uniprot: P01127
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Szabo, A., et al. Stroke 38 (8), 2247-2253 (2007)
2. Guida, T., et al. Clin. Cancer Res. 13 (11), 3363-3369 (2007)
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