THUNDER Phospho-c-Met (Y1234/Y1235) + Total c-Met TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-c-Met (Y1234/1235) and Total c-Met in cell lysates.

Components: Eu-phospho-MET (20 µL), Acc-phospho-MET (80 µL), Eu-Total MET (5 µL), Acc-Total MET (20 µL), MET control lysate (500 µL), 5X Lysis Buffer 5 (5 mL), 10X TR-FRET Detection buffer (250 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Phospho c-Met (Y1234/1235) and Total c-Met .

Assay Target Class: Kinase.

SwissProt: P08581.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-METPT-500
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-METPT-500
• Green Labware: No
• Package Unit: 500 Points (400 point for phospho; 100 Points for total)
• Quantity Unit: PAK
• Reactivity: Human
• Application: Cell-based Assay
• Human Gene ID: 4233