THUNDER Phospho-p70 S6K (T389) + Total p70 S6K TR-FRET Cell Signaling Assay Kit

Description: Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p70 S6K (T389) and Total p70 S6K in cell lysates.

Components: Eu-phospho-P70S6 (20 µL), Acc-phospho-P70S6 (80 µL), Eu-Total P70S6 (5 µL), Acc-Total P70S6 (20 µL), P70S6 control lysate (500 µL), 5X Lysis Buffer 3 (5 mL), 10X TR-FRET Detection buffer (250 µL).

Assay Type: Semi-quantitative; homogeneous (no-wash) sandwich immunoassay.

Assay Format: Half-area 96-well or low-volume 384-well plate.

Assay Target: Phospho-p70 S6K (T389) and Total p70 S6K .

Assay Target Class: Kinase.

SwissProt: P23443.

Detection method: TR-FRET.

Sample type: Cell lysates (adherent or suspension cells).

• SKU: BIXKIT-P70S6KPT-500
• Manufacturer: BioAuxilium
• Manufacturer SKU: KIT-P70S6KPT-500
• Green Labware: No
• Package Unit: 500 Points (400 Points for phospho; 100 Points for total)
• Quantity Unit: PAK
• Reactivity: Human
• Application: Cell-based Assay
• Human Gene ID: 6198