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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: A 25 µl kinase reaction is conducted in a buffer containing 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 5 mM MgCl2
Description: Complex of N-terminal GST-tagged recombinant full-length human p110α (GenBank Accession No. NM_006218), and recombinant full length, human p85α (no tag) (GenBank Accession No. NM_181523). Coexpressed in a Baculovirus infected Sf9 cell expression system. p110α MW=151 kDa, p85α MW=84 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol, and 3 mM DTT.
Genbank: NM_006218 (p110α), NM_181523 (p85α)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal GST-tag(p110α), no tag on p85α
Uniprot: p110α: P42336; p85α: P27986
Unit Definition: A unit of p110α/p85α kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Vogt, PK et al., Virology. 344(1):131-(2006)
2. Bader, AG et al., Nat Rev Cancer. Dec 5(12):921-9 (2005)
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