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Encompassing Amino Acids: PIK3CG 2-1102 (end), PIK3R5 2-880 (end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Complex of N-terminal His-tag recombinant human p110γ (PIK3CG), (GenBank Accession No. NM_002649) a.a. 2-1102 (end), and N-terminal GST-tag recombinant human PIK3R5 (GenBank Accession No. NM_001142633) a.a. 2 -880 (end). Co-expressed in a Baculovirus infected Sf9 cell expression system. p110 MW=127 kDa, PIK3R5 MW=124 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 3 mM DTT, 20% glycerol.
Genbank: NM_002649 (p110γ), NM_001142633 (PIK3R5)
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag (p110γ), N-terminal GST-tag (PIK3R5)
Uniprot: p110γ: P48736; PIK3R5: Q8WYR1
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Shymanets, A. et al., Biochem. J. 2012 Feb 1,441(3): 851-8.
2. Brazzatti, J.A., et al., Oncogene. 2012 May 3,31(18):2350-61.