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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 µM ATP, 0.01% Brij-35, and 2 µM substrate (SER/THR 14, Invitrogen) at room temperature for 1 hour.
Description: cGMP-dependent Human PKG1α (GenBank Accession No. NM_001098512), full length with N-terminal His-tag, MW = 80 kDa, expressed in Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT.
Genbank: NM_001098512
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q13976
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Sharma,A.K.et al., J. Biol. Chem. 283 (47), 32860-32869 (2008).
2. Pohler,D.et al., FEBS Lett. 374 (3), 419-425 (1995).
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