Products from BPS Bioscience require a minimum order value above 400€
Encompassing Amino Acids: 22-441(end)
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: Enzyme reaction is conducted using liposomally incorporated Red/Green BODIPY PC-A2 (5 µM, Invitrogen) as a substrate in a reaction buffer (50 mM Tris, pH 8.8, 100 mM NaCl, 1 mM CaCl2). Reaction progress was monitored at ex480/em533 continuously at room temperature.
Description: Human PLA2G7, (GenBank Accession No. NM_005084), a.a. 22-441(end), with C-terminal His-tag, MW=49.2 kDa, expressed in a mammalian cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol
Genbank: NM_005084
Storage Stability: At least 6 months at -80°C.
Tags: C-terminal His-tag
Uniprot: Q13093
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Berg M., et al., Mol Cancer. 9:100. (2010).
2. Kopec AK., et al., Toxicol Appl Pharmacol.256(2):154-67. (2011).