Immunogen: Synthetic peptide (CQSYPIGDDEESESD-OH) from the C-terminus of rat VMAT2 conjugated to BSA
Concentration: n/a
Storage buffer: Lyophilized; reconstitute in 100 µl dist. water
Additional info: The vesicular monoamine transporter is responsible for the vesicular uptake of monoamines, like dopamine, norepinephrine, epinephrine, serotonin and histamine. The antiserum recognizes monoaminergic neurons of the CNS, the ECL-cells of the stomach, as well as enteric nerve fibers.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes staining. In Western blot experiments using a crude synaptic vesicle fraction of rat brain, the 70 kDa of anti-VMaT2 is recognized (suggested dilution 1:500).Positive control: frozen sections of rat small intestine.
References: Carballo-Carbajal, I. et al. Nat.Commun. 2019;10: 973
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