Immunogen: Synthetic met-enkephalin (Sigma), conjugated to BSA
Concentration: n/a
Storage buffer: Lyophilized; reconstitute in 100 µl dist. water
Additional info: Enkephalins are small peptides derived from large precursers (pro-enkephalin A and B) containing multiple enkephalin copies. They are the most abundant opioid peptides in the body and are widely distributed in the brain and the peripheral nervous system and occur also in the adrenal medulla. Several types of neuroendocrine tumors, incl. pheochromocytomas, neuroblastomas and bronchial and gastrointestinal endocrine tumors, produce enkephalin.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes the staining, while beta-endorphin does not. Cross-reacts with leu-enkephalin.Positive control: Frozen sections of cat or pig small intestine.
References: Kirchgessner, A. L. et al. Neurol.1989; 285, 38–53 /Thesleff, P. et al. Gut 1987;28:1298–301/Ekblad, E. et al. Neuroscience1994; 63: 233–248
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