Immunogen: Synthetic rat PRP (Pensinsula), conjugated to BSA
Concentration: n/a
Storage buffer: Lyophilized; reconstitute in 100 µl dist. water
Additional info: Pituitary adenylate cyclase activating peptide (PACAP), originally isolated from ovine hypothalamus, belongs to the VIP-family of peptides. PACAP-related peptide (PRP) is a 29-amino acid peptide, which is produced together with PACAP-27 and PACAP-38 when PreproPACAP is being processed. PRP is abundant in the brain, but can be also found in the respiratory and gastrointestinal tracts.
Absorption with 10-100 ug immunogen per ml diluted antiserum abolishes the staining, while PACAP-27, PACAP-38, VIP, PHI, CRF, oxytocin and vasopressin do not.
Positive control: Stefanini-fixed frozen sections of rat duodenum, fundus or antrum.