Products from BPS Bioscience require a minimum order value above 400€
Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Background: Prolyl oligopeptidase (POP), also known as prolyl endopeptidase(PREP), is a cytosolic serine peptidase involved in the maturation and degradation ofpeptide hormones and neuropeptides.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The Fluorogenic Prolyl OligoPeptidase (POP) Assay Kit is a complete assay system designed to measure activity of the purified POP enzyme. The Fluorogenic POP Activity Kit eliminates the dealing with radioactive materials and chromatography in traditional assays. Purified human recombinant POP is included in the kit as a positive control. Using this kit, only one simple step, in which the fluorometric substrate is incubated with purified POP, is needed to analyze the POPactivity level. The resulting fluorescent product can then be easily measured with a microtiter-plate fluorimeter.
Storage Stability: At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: Supplied in a convenient 96-well format, with purified POP enzyme, substrate, and assay buffer for 100 enzymereactions.
Uniprot: P48147
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Myohanen T.T. et al., Neurochem. Res. 32(8), 1365-1374 (2007).
2. Garcia-Horsman J.A. et al., Scand. J.Gastroenterol. 42 (5), 562-571 (2007).
English
Deutsch
