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Encompassing Amino Acids: 31-496
Applications: Useful for the cleavage of C-terminal amino acids linked to proline in peptides, for example, angiotension II, III and des-Arg9-bradykinin.
Background: PrCP is a lysosomal prolylcarboxypeptidase that cleaves C-terminal amino acids linked to proline. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. This enzyme has been shown to be an activator of the cell matrix-associated prekallikrein. PrCPs role in angiotension II cleavage, which regulates blood pressure and electrolyte balance, suggests that PrCP may be involved in hypertension.
Description: Human recombinant Lysosomal Pro-X carboxypeptidase, also known as angiotensinase C (GenBank Accession #NM_005040), a.a. 31-496 with C-terminal His-tag, MW=53 kDa, expressed in HEK293 cells.
Format: Aqueous buffer solution
Formulation: 40 mM Tris pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT, 200 mM imidazole
Genbank: NM_005040
Storage Stability: Stable at least 6 months from date of receipt, when stored as directed
Tags: C-terminal His-tag
Uniprot: P42785
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Tan, F., et al. 1993. J Biol Chem 268 (22): 16631-8.
2. Yang, H.Y., et al. 1968. Nature 218 (5148): 1224-6.
3. Wang, L., et al. 2006. Am. J. Obstet. Gynecol. 195 (1): 162-71.
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