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Encompassing Amino Acids: 2-400
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: For standard, H3, 1-21 K4 (2Me)-Biotin was coated onto a neutravadin plate at varying concentrations. For test lots, H3,1-21-Biotin was coated onto a neutravadin plate at 0.4 µg/ml. Reaction mixture contained 50 mM Tris buffer pH 8.8, 5 mM MgCl2, 4 mM DTT, 0.05% Tween-20, 40 µM S-adenosylmethionine, and various amounts of PRDM9 incubated for 1 hour at room temperature. Followed by 1 hour incubation with primary antibody against H3 K4 (2Me), and 30 minute incubation with secondary antibody. Luminescence was developed with HRP substrate A and B (Pierce).
Description: Human PRDM9, also known as PR domain zinc finger protein 9, MEISETZ, and PR/SET domain 9, GenBank Accession No. NM_020227, a.a. 2-400, with an N-terminal GST-tag, expressed in an E. coli expression system. MW = 73 kDa.
Format: Aqueous buffer solution.
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20mM glutathione, 3mM DTT and 20% glycerol
Genbank: NM_020227
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal FLAG-Tag
Uniprot: Q9NQV7
Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Biosafety Level: Not applicable (BSL-1)
References: 1. Baudat, F., et al., Science. 2010 Feb; 327(5967): 836-40.
2. Parvanov, E., et al., Science. 2010 Feb; 317(5967): 835.
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