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Applications: Great for studying enzyme kinetics and HTS applications.
Background: PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Description: The PRMT5 TR-FRET Assay Kit is designed to measure PRMT5 activity in a homogeneous 384 reaction format. PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The key to the PRMT5 TR-FRET Assay Kit is a highly specific antibody that recognizes methylated substrate.
Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Supplied As: The PRMT5 TR-FRET Assay Kit comes in a convenient format, with histone H4 peptide substrate, a Eu-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, FRET detection buffer, dye-labeled acceptor, and purified PRMT5 for 384 enzyme reactions.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Yang, Y., Bedford, M.T. 2013. Nat Rev Cancer. 13(1):37-50.