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Encompassing Amino Acids: 2-845
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Description: Human Protein Arginine Methyltransferase 9 (PRMT9), GenBank Accession No. NM_138364, a.a. 2-845, with an N-terminal FLAG-tag and a C-terminal Strep-tag, expressed in an SF9 insect cell expression system. MW = 97 kDa.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 80 μg/ml FLAG peptide.
Genbank: NM_138364
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: N-terminal FLAG-Tag, C-terminal Strep-Tag
Uniprot: Q6P2P2
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1.Cook, J, et al., Biochem. Biophys. Res. Commun. 2006 Apr; 342(2):475-481.2.Yanzhong, Y., et al., Nature. 2014 Jun; 6: 267-273.