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Encompassing Amino Acids: full length
Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Assay Conditions: ADP-Glo assay with 0.1 mg/ml poly-(Glu4:Tyr) as a substrate and 100 mM ATP at final concentration. Reaction was done for 60 minutes at 30°C. ADP-Glo reagents were added for 45 minutes at RT, and Kinase detection reagents for 55 minutes at RT, followed by luminescence detection.
Description: Human PYK2 (GenBank Accession No. U33284), full length with N-terminal His-tag, MW=120 kDa, expressed in a Baculovirus infected Sf9 cell expression system.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Genbank: U33284
Host Cell Line: Sf9 cells
Storage Stability: At least 6 months at -80°C.
Tags: N-terminal His-tag
Uniprot: Q14289
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Cain R.J. et al., J Cell Biol. 2010 Mar 22,188(6):863-76.
2. Collins M. et al., Mol Immunol. 2010 May,47(9):1665-74.