Background: Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.
Immunogen: Synthetic peptide derived from human DICER1 protein.
Purification Method: Antigen Immunoaffiinity Purification.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Zhang, H., et al. ‘Single processing center models for human Dicer and bacterial RNase III.’ Cell, 118, 57-68 (2004)
2. Meister, G., et al. ‘Identification of novel argonaute-associated proteins.’ Curr. Biol., 15, 2149-2155 (2005)
3. Haase, A.D., et al. ‘TRBP, a regu1ator of cellular PKR and HIV-1 virus expression, interacts with Dicer and functions in RNA silencing.’ EMBO Rep., 6, 961-967 (2005)
4. Kok, K.H., et al. ‘Human TRBP and PACT directly interact with each other and associate with dicer to facilitate the production of small interfering RNA.’ J. Biol. Chem., 282, 17649-17657 (2007)
5. Melo, S.A., et al. ‘A TARBP2 mutation in human cancer impairs microRNA processing and DICER1 function.’ Nat. Genet., 41, 365-370 (2009).
UniProt: Q9UPY3.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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