Background: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus (1). DNase II causes both chromatin condensation and DNA fragmentation (1). The genes encoding human (2-4), porcine (4), and murine (5) DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (a) and 12 kDa (b) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation (3). DNase II is ubiquitously expressed in human tissues.
Positive Control: Tissue lysates from human spleen.
Immunogen: A synthetic peptide corresponding to amino acids 347 to 360 of human DNase II precursor (2-4).
Purification Method: Antigen Immunoaffiinity Purification.
Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative.
References: 1. Condensed matter in cell death. Zamzami, N., et al. Nature 1999, 401, 127-8
2. Molecular cloning of the cDNA encoding human deoxyribonuclease II. Yasuda, T., et al., J.
Biol. Chem. 1998, 273, 2610-6
3. The cloning and expression of human deoxyribonuclease II. A possible role in apoptosis. Krieser, R.J. and Eastman, A. J. Biol. Chem. 1998, 273, 30909-14
4. Cloning of cDNAs encoding porcine and human DNase II. Shiokawa, D., et al., Biochem. Biophys. Res. Commun. 1998, 247, 864-9
5. Molecular cloning and characterization of human and murine DNase II. Baker, K.P., et al., Gene 1998, 215, 281-9.
UniProt: O00115.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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