Background: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD)- containing adapter molecules and members of the ICE/CED-3 protease family. Caspases-8 (FLICE) and -10 (FLICE2) are two pivotal members in the ICE/CED-3 protease family. FLICE-inhibitory proteins were identified in virus and human and designated v-FLIPs and FLIP, respectively1,2. The human FLIP was also cloned by several labs independently and termed Casper, I-FLICE, FLAME-1, CASH and CLARP3-7. FLIP contains two death effector domains (DEDs) and a caspase-like domain. FLIP interacts with adapter protein FADD and caspase-8 and Ð10, and potently inhibits apoptosis induced by all known death receptors. Four splice variants of c-FLIPs have been identified and termed FLIP , , , and , respectively8.
Immunogen: Antibody raised against a synthetic peptide corresponding to amino acids 2 to 18 of the human FLIP protein. This sequence is identical in all known FLIP splice variants.
Purification Method: Antigen Immunoaffiinity Purification.
Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative.
References: 1. Thome, M., et al. Viral FLICE-inhibitory proteins (FLIPs) prevent apoptosis induced by death receptors. Nature 1997, 386, 517-521
2. Irmler, M., et al. Inhibition of death receptor signals by cellular FLIP. Nature 1997, 388, 190-195
3. Shu, H.B., et al. Casper is a FADD- and caspase-related inducer of apoptosis. Immunity 1997, 6, 751-763
4. Hu, S., et al. I-FLICE, a novel inhibitor of tumor necrosis factor receptor-1- and CD-95-induced apoptosis. J. Biol. Chem. 1997, 272, 17255-17257
5. Srinivasula, S.M., et al. FLAME-1, a novel FADD-like anti-apoptotic molecule that regulates Fas/TNFR1-induced apoptosis. J. Biol. Chem. 1997, 272, 18542-18545
6. Goltsev, Y.V., et al. CASH, a novel caspase homologue with death effector domains. J. Biol. Chem. 1997, 272, 19641-19644
7. Inohara, N., et al. CLARP, a death effector domain-containing protein interacts with caspase-8 and regulates apoptosis. Proc. Natl. Acad. Sci. USA 1997, 94, 10717-10722
8. Wallach, D. Apoptosis. Placing death under control. Nature 1997, 388, 123
Product Specific References:
1. Mirandola, P., et al. 'Activated human NK and CD8+ T cells express both TNF-related apoptosis-inducing ligand (TRAIL) and TRAIL receptors but are resistant to TRAIL-mediated cyotoxicity.' Blood, 2004, 104, 2418-2424.
UniProt: Q9UKL3.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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