Background: Germline mutations of the LKB1 (STK11) tumor suppressor gene can lead to Peutz-Jeghers syndrome (PJS) and a marked predisposition to cancer. LKB1 encodes a serine/threonine kinase that is inactivated in PJS. The tumor suppressor protein PTEN is a LKB1-interacting protein. Several point mutations in LKB1 are associated with PJS and may disrupt the interaction with PTEN suggesting that the loss of this interaction might contribute to PJS. PTEN and LKB1 are predominantly cytoplasmic and nuclear, respectively, the interaction of the two leads to a cytoplasmic relocalization of LKB1. PTEN is a substrate of the kinase LKB1 in vitro.
Positive Control: Lung, Uterus
Immunogen: Synthetic peptide derived from the human LKB1/STK11 serine/threonine kinase protein.
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Formulation: Purified IgG in phosphate buffered saline and 0.08% Sodium Azide
References: 1. Shaw RJ, Bardeesy N, Manning BD, Lopez L, Kosmatka M, DePinho RA, Cantley LC.The LKB1 tumor suppressor negatively regulates mTOR signaling. Cancer Cell. 2004 Jul;6(1):91-9. 2. Shaw RJ, Kosmatka M, Bardeesy N, Hurley RL, Witters LA, DePinho RA, Cantley LC. The tumor suppressor LKB1 kinase directly activates AMP-activated kinase and regulates apoptosis in response to energy stress. Proc Natl Acad Sci U S A. 2004 Mar 9;101(10):3329-35. Epub 2004 Feb 25. 3. Karuman P, Gozani O, Odze RD, Zhou XC, Zhu H, Shaw R, Brien TP, Bozzuto CD, Ooi D, Cantley LC, Yuan J. The Peutz-Jegher gene product LKB1 is a mediator of p53-dependent cell death. Mol Cell. 2001 Jun;7(6):1307-19. 4. Jenne D.E., Reimann H., Nezu J., Friedl W., Loff S., Jeschke R., Mueller O., Back W., Zimmer M.; Peutz-Jeghers syndrome is caused by mutations in a novel serine threonine kinase.; Nat. Genet. 18:38-43(1998).5. Bignell G.R., Barfoot R., Seal S., Collins N., Warren W., Stratton M.R.; Low frequency of somatic mutations in the LKB1/Peutz-Jeghers syndrome gene in sporadic breast cancer.; Cancer Res. 58:1384-1386(1998).6. Strausberg R.L., Feingold E.A., Grouse L.H., Derge J.G., Klausner R.D., Collins F.S., Wagner L., Shenmen C.M., Schuler G.D., Altschul S.F., Zeeberg B., Buetow K.H., Schaefer C.F., Bhat N.K., Hopkins R.F., Jordan H., Moore T., Max S.I., Wang J., , Marra M.A.; Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).7. Nakagawa H., Koyama K., Miyoshi Y., Ando H., Baba S., Watatani M., Yasutomi M., Matsuura N., Monden M., Nakamura Y.; Nine novel germline mutations of STK11 in ten families with Peutz-Jeghers syndrome.; Hum. Genet. 103:168-172(1998).8. Hemminki A., Markie D., Tomlinson I., Avizienyte E., Roth S., Loukola A., Bignell G., Warren W., Aminoff M., Hoeglund P., Jaervinen H., Kristo P., Pelin K., Ridanpaeae M., Salovaara R., Toro T., Bodmer W., Olschwang S., Olsen A.S., , Aaltonen L.A.; A serine/threonine kinase gene defective in Peutz-Jeghers syndrome.; Nature 391:184-187(1998).9. Westerman A.M., Entius M.M., Boor P.P.C., Koole R., de Baar E., Offerhaus G.J.A., Lubinski J., Lindhout D., Halley D.J.J., de Rooij F.W.M., Wilson J.H.P.; Novel mutations in the LKB1/STK11 gene in Dutch Peutz-Jeghers families.; Hum. Mutat. 13:476-481(1999).
UniProt: Q15831
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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