Background: Lysophospholipid acyltransferases (LPLATs) catalyse the addition of fatty acyl moieties to the glycerol backbone of lysophospholipids . In addition to playing a crutial role in the synthesis of structural membrane components the LPLATs are also implicated in cellular signalling responses for cytokines, growth factors and other agonists. Cellular signalling through the interleukin 1 receptor in human mesangial cells and EL-4 cells proceeds by the activation of lysophosphatidic acid acyltransferase (LPAAT). Activation of LPAAT by interleukin 1 results in the generation of unsaturated phosphatidic acid species, that are crucial to the generation of diacylglycerol and interleukin 1 signalling. LPLATs are also involved in signalling for increased interleukin 2 synthesis through the T cell antigen receptor. Activation of T-cells via anti-CD3 stimulation has been shown to increase incorporation of polyunsaturated fatty acids into phosphatidylcholine via lysophosphatidylcholine acyltransferase. Activation of this enzyme is essential for the sustained activation and translocation of protein kinase C.
Positive Control: Human brain lysate (Cat. No. X1633C)
Immunogen: Synthetic peptide derived from the LPAAT- protein
Purification Method: Antigen Immunoaffiinity Purification
Concentration: See vial for concentration
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
References: [1] Eberhardt C., Gray P.W., Tjoelker L.W.; Human lysophosphatidic acid acyltransferase. cDNA cloning, expression, and localization to chromosome 9q34.3.; J. Biol. Chem. 272:20299-20305(1997).[2] Stamps A.C., Elmore M.A., Hill M.E., Kelly K., Makda A.A., Finnen M.J.; A human cDNA sequence with homology to non-mammalian lysophosphatidic acid acyltransferases.; Biochem. J. 326:455-461(1997).[3] West J., Tompkins C.K., Balantac N., Nudelman E., Meengs B., White T., Bursten S., Coleman J., Kumar A., Singer J.W., Leung D.W.; Cloning and expression of two human lysophosphatidic acid acyltransferase cDNAs that enhance cytokine-induced signaling responses in cells.; DNA Cell Biol. 16:691-701(1997).[4] Leung D.W., Tompkin C.K., West J.; Submitted (MAY-2001) to the EMBL/GenBank/DDBJ databases.[5] Strausberg R.L., et al Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.; Proc. Natl. Acad. Sci. U.S.A. 99:16899-16903(2002).[6] Garg A.; Acquired and inherited lipodystrophies.; N. Engl. J. Med. 350:1220-1234(2004).[7] Agarwal A.K., Arioglu E., de Almeida S., Akkoc N., Taylor S.I., Bowcock A.M., Barnes R.I., Garg A.; AGPAT2 is mutated in congenital generalized lipodystrophy linked to chromosome 9q34.; Nat. Genet. 31:21-23(2002).
UniProt: O15120
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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