Background: Nuclear factor kappa B (NFkB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NFkB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNF alpha LPS and mitogens. A serine/threonine protein kinase which mediates NFkappa;B activation by IL-1, TNF alpha; and CD95 was identified recently and designated NIK (for NF kappa;B inducing kinase) . NIK is an activator of I kappa B kinase alpha; and beta; (IKK alpha; and IKK beta;) (2-5). Therefore, NIK is a key molecule in the NF kappa;B signaling pathway leading to the induction of a variety of gene expression in response to proinflammatory cytokines and bacteria products.
Positive Control: Weakly expressed in testis, small intestine, spleen, thymus, peripheral blood leukocytes, prostate, ovary and colon. Located in the cytoplasm.
Immunogen: Rabbit polyclonal NIK antibody was raised against a peptide corresponding to amino acids 931 to 947 of human NIK.
Purification Method: Antigen Immunoaffiinity Purification.
Formulation: Provided as solution in phosphate buffered saline with 0.02% sodium azide.
References: 1) Malinin NL, Boldin MP, Kovalenko AV, Wallach . MAP3K-related kinase involved in NF-kappa B induction by TNF, CD95 and IL-1.Nature 1997;385:540-544.
2) Regnier CH, Song HY, Gao X, Goeddel DV, Cao Z, Rothe M. Identification and characterization of an I kappa;B kinase..Cell 1997;90:373-383.
3) Woronicz JD, Gao X, Cao Z, Rothe M, Goeddel DV. I kappa B kinase-beta;: NF-kappa B activation and complex formation with I kappa;B kinase alpha; and NIK. Science 1997;278:866-869
4) Ling L, Cao Z, Goeddel D. NF kappa B-inducing kinase activates IKK alpha by phosphorylation of Ser-176. Proc Natl Acad Sci USA 1998;95:3792-3797.
UniProt: Q99558.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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