Background: As first published in 1998 by Sakurai et al,, the initial neuropeptides Orexin A and Orexin B were shown to be important in the regulation of appetite. Continued research now suggests that these peptides also influence a wide range of physiological and behavioral processes such as increased EFG arousal and wakefulness and locomotor activity. In addition to the orexins , two G-protein coupled receptors, designated orexin receptor-1 and orexin receptor-2, have been identified which presumably mediate the signaling events from these neuropeptides1. Recently Orexin Receptors have been found to activate extracellular signal-regulated kinases in CHO cells, namely ERK phosporylation, through Ca2+ influx..2. Further studies seem to indicate a mechanism for orexin receptor mediated cell death and suggest a pathway from GPCRÕs to apoptosis throughthe MAPK pathways.3
Positive Control: membrane
Immunogen: Synthetic peptide made to the N terminal of human Orexin Receptor 1
Purification Method: Antigen Immunoaffiinity Purification
Concentration: See vial for concentration
Formulation: Immunoaffinity antibody povided as solution in phosphate buffered saline with 0.08% sodium azide
References: 1. Rodger, R. J. et al, Orexins and Appetite Regularion, Neuropeptides,(2002) Vol 36, pp.303-325.2.Ammoun, Sylvia et al., OX1 Orexin Receptors Activate Extracellular Signal-Regulated Kinase in Chinese Hamster Ovary Cells via Multiple Mechanisms: The Role of Ca2+ Influx in OX1 Receptor Signaling. Molecular Endocrinology, 2006, Issue 20, vol 1, pp.80-993. Ammoun, Sylvia et al. G- protein couples OX1 Orexin/hcrtr-1 Hypocretin Recptors Induce Caspase-dependent and -independent Cell Death through p38 Mitogen-/Stress-activated Protein Kinase, J. Biol. Chem, January 13, 2006, Vol 281, Issue 2, pp834-843,
UniProt: O43613
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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