Background: PHLPP1 is a 1717 aa protein with phosphatase activity that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and suppression of tumor growth. Controls the phosphorylation of AKT2 and AKT3 more efficiently than that of AKT1. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. May act as a negative regulator of K-Ras signaling in membrane rafts.
Immunogen: Synthetic peptide derived from the human PHLPP1 protein.
Purification Method: Antigen Immunoaffinity Purification.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Gao, T., et al. ‘PHLPP: a phosphatase that directly dephosphorylates Akt, promotes apoptosis, and suppresses tumor growth’ Mol. Cell, 18, 13-24, 2005
2. Bragnard, J., et al. ‘PHLPP and a second isoform, PHLPP2, differentially attenuate the amplitude of Akt signaling by regulating distinct Akt isoforms.’ Mol. Cell, 25, 917-931, 2007
3. Gao, T., et al. ‘The phosphatase PHLPP controls the cellular levels of protein kinase C.’ J. Biol. Chem., 283, 6300-6311, 2008.
UniProt: O60346.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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