Background: SMO is a G protein-coupled receptor (GPCR) that putatively associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. The binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). In basal cell carcinoma there are two somatic mutations SMO, D473-> H473 and R562 ->Q562.
Positive Control: Brain
Immunogen: Synthetic peptide derived from the human SMO protein
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
References: 1. Stone, D.M., et al. The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog.; Nature 384:129-134(1996).2. Xie, J., et al. Activating Smoothened mutations in sporadic basal-cell carcinoma.; Nature 391:90-92(1998).3. Liu, Y., et al. 'Fused-Costal2 protein complex regulates Hedgehog-induced Smo phosphorylation and cell-surface accumulation.' Genes Dev. 2007 Aug 1;21(15):1949-63. 4. Moraea, R.C., et al. 'Constitutive activation of smoothened (SMO) in mammary glands of transgenic mice leads to increased proliferation, altered differentiation and ductal dysplasia.' Development. 2007 Mar;134(6):1231-42. 5. Ruiz-Gomez, A., et al. 'The cell biology of Smo signalling and its relationships with GPCRs.' Biochim Biophys Acta. 2007 Apr;1768(4):901-12.
UniProt: Q99835 (Human)
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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