Background: Tumor-necrosis factor- is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF- is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF- . TNF- converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately (1-3). TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGF , the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP) (4,5). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association. (6).
Positive Control: HeLa and Jurkat whole cell lysates.
Immunogen: Synthetic peptide corresponding to amino acids 807 to 823 of the human TACE protein. Immunogen sequence differs from the mouse and rat sequences by one amino acid.
Purification Method: Antigen Immunoaffiinity Purification.
Formulation: Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative.
References: 1. Black, R.A., et al. A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature 1997, 385, 729-733
2. Moss, M.L., et al. Cloning of a disintegrin metalloproteinase that processes precursor tumour-necrosis factor-alpha. Nature 1997, 385, 733-736
3. Mizui, Y., et al., cDNA cloning of mouse tumor necrosis factor-alpha converting enzyme (TACE) and partial analysis of its promoter. Gene 1999, 233, 67-74
4. Peschon, J.J., et al. An essential role for ectodomain shedding in mammalian development. Science 1998, 282, 1281-4
5. Buxbaum, J.D., et al. Evidence that tumor necrosis factor alpha converting enzyme is involved in regulated alpha-secretase cleavage of the Alzheimer amyloid protein precursor. J. Biol. Chem. 1998, 273, 27765-27767
6. Giegel, D.A. and Chaplin, D.D. Targets in cytokine activation. Agents Actions Suppl. 1998, 49, 1-3
7. Blaydon, D.C, et al. Imflammatory Skin and Bowel Disease Linked to ADAM17 Deletion N Engl J Med, 2011, 365, 1502-1508.
UniProt: P78536 (Human)
Q9Z0F8 (Mouse)
Q9Z1K9 (Rat)
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Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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