Clone: Polyclonal
Background: The reactivity of the antiserum is restricted to alpha-2 macroglobulin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified alpha-2 macroglobulin. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence alpha-2 macroglobulin in mouse serum or other body fluids and to determine its concentration. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays, but this does not exclude such use if proper controls are included.
Source: Alpha-2 macroglobulin ids the major protein in the alpha-2 region of mouse serum after separation by electrophoresis. It is a large polymer and a strong antigen. It forms about one-third in the total of the alpha-2 globulins. The protein is isolated by ammonium sulphate precipitation, ion exchange chromatography, gel filtration and preparative starch block electrophoresis. In the purification of alpha-2 macroglobulin the activation of kallikrein is avoided, since it forms an irreversible bond with aplpha-2 macroglobulin, thus contaminating the final immunogen. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Precipitating polyclonal Rabbit antiSerum to Mouse alpha-2 macroglobulin
Formulation: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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