Background: The Kv1.3 potassium channel is a voltage-gated channel protein which belongs to the delayed rectifier class and to the Shaker potassium channel subfamily which includes Kv1.1, Kv1.2, Kv1.4 and Kv1.5. Potassium channels are mainly found in plasma membranes but are not generally distributed over the cell surface. Potassium channels catalyze the rapid permeation of potassium ions while rejecting biologically abundant potential competitors such as sodium, calcium and magnesium. Ion selectivity and high through put rate of potassium channels is accomplished by precise co-ordination of dehydrated potassium by the protein and multiple ion occupancy within the permeation pathway. All potassium channels carry out the formation of a transmembrane leak specific for potassium ions. Since cells almost universally maintain cytoplasmic potassium concentrations higher than those extracellularly, the opening of a potassium channel implies a negative ongoing change in electrical voltage across the cell membrane. This may result in termination of the action potential of electrically excitable cells including nerve, muscle and pancreatic beta cells. In nonÐexcitable cells, potassium channels play important roles in the cellular potassium recycling required for electrolyte balance effected by the renal epithelium.
Positive Control: Rat brain lysate.
Immunogen: Synthetic peptide derived from the rat Kv1.3 potassium channel conjugated to KLH.
Purification Method: Ammonium Sulfate Precipitation.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Stuhmer, W et.al. 'Molecular basis of functional diversity of voltage-gated potassium
channels in mammalian brain.' EMBO J. 8 (11), 3235-3244 (1989)
2. Swanson, R et.al. 'Cloning and expression of cDNA and genomic clones encoding threedelayed rectifier potassium channels in rat brain.' Neuron 4 (6), 929-939 (1990)
3. Douglass, J et.al. 'Characterization and functional expression of a rat genomic DNA
clone encoding a lymphocyte potassium channel' J. Immunol. 144 (12), 4841-4850 (1990)
4. Chang, M.C. et.al 'Regulation of Kv1.3 channels in activated human T lymphocytes by Ca(2+)-dependent pathways.' Cell Physiol Biochem.11(3):123-34. 2001
5. Butchason,I., et. al. 'Angular methoxy-substituted furo- and pyranoquinolinones as blockers of the voltage-gated potassium channel Kv1.3.' J. Med. Chem. 44(8):1249-56 (2001).
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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