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Description: Human Receptor Activator of Nuclear Factor κ B Ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 and TRANCE, (Genbank Accession No. NM_003701), a.a. 64-245, with an N-terminal His-tag, expressed in a HEK293 cell expression system. MW = 23 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Format: Aqueous buffer solution
Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Genbank: NM_003701
Host Cell Line: HEK293
Purity: ≥70%
Storage Stability: At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to ≥ 100 µg/ml in PBS + glycerol and stored at -80°C.
Tags: N-terminal His-Tag
Uniprot: O14788
Warnings: Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
Biosafety Level: Not applicable (BSL-1)
References: 1.Yasuda, H., J. American Chemical Society., 1998 March; 126(4): 3597-3602.2.Takayanagi H, et al. Dev Cell. 2002 Dec;3(6):889-901.