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Applications: Useful for studying RAS/RAF binding study as well as inhibitor screening for RAS/RAF interaction.
Assay Conditions: GppNHp (or GDP) loaded His-tag KRAS(G12C) at various concentrations (BPS Bioscience #100641 and BPS Bioscience #100640) was incubated with 2.5 nM GST-tag RBD-RAF1 for 1 hour at room temperature. KRAS(G12C)/RBD-RAF1 binding was analyzed by AlphaScreening using GSH-Acceptor beads and Nickle Donor beads (PerkinElmer).
Description: Recombinant Human Raf-1 proto-oncogene, serine/threonine kinase (RAF1), encompassing amino acids 50-140. This construct contains an N-terminal GST-tag followed by a PreScission site to facilitate removal of GST-tag. The recombinant protein was affinity purified.
Format: Aqueous buffer solution
Formulation: 40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol.
Genbank: NM_002880
Storage Stability: At least 6 months at -70°C.
Supplied As: Aqueous buffer solution.
Tags: N-terminal GST-tag
Uniprot: P04049
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Rooij, J., et al., Oncogene. 1997;14:623-625.
2. Bhaswati, P., et al., Nature Genetics. 2007;39:1007-1012.
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