Recombinant Human PBEF/NAMPT Protein (His Tag)

Abbreviation: PBEF;NAMPT

Target Synonym: Pre-B cell-enhancing factor;Nicotinamide phosphoribosyltransferase;NAmPRTase;Nampt;Pre-B-cell colony-enhancing factor 1;Visfatin;NAMPT;PBEF;PBEF1

Target Species: Human

Expression Host: E.coli

Fusion Tag: N-His

UNIProt ID: P43490

Background: Pre-B cell colony enhancing factor (PBEF) was originally identified as a cytokine that potentiated the clonal expansion and differentiation of pre-B cells; but it is also acknowledged to be the ubiquitous intracellular enzyme nicotinamide phosphoribosyltranferase (NAMPT) and the adipokine “visfatin”. PBEF is constitutively expressed in the fetal membranes where its greatest expression is in the amnion. It has intracellular and extracellular forms. Most of the intracellular functions of PBEF are due to its role as a Nampt which can induce angiogenesis through upregulation of VEGF and VEGFR and secretion of MCP-1. Extracellular PBEF has been shown to increase inflammatory cytokines; such as TNF-α; IL-1β; IL-16; and TGF-β1. PBEF also increases the production of IL-6; TNF-α; and IL-1β in CD14+ monocyctes; macrophages; and dendritic cells; enhances the effectiveness of T cells.

Sequence: Met1-His491

Purity: > 90 % as determined by reducing SDS-PAGE.

Formulation: Lyophilized from a 0.2 um filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method.

• SKU: ELSPDEH100011-100
• Manufacturer: Elabscience Biotechnology
• Manufacturer SKU: ELA-PDEH100011-100
• Green Labware: No
• Package Unit: 100 µg
• Quantity Unit: STK