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Encompassing Amino Acids: H2A 2-130(end), H2B 2-126(end), H3 2-137(end), H4 2-103(end)
Applications: Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications.
Description: Human recombinant nucleosomesconsisting of 147 bp DNA (PCR) and 2 moleculeseach of histones H2A (a.a. 2-130(end)), H2B(a.a. 2-126(end)), H3 (a.a. 2-137(end)), and H4(a.a. 2-103(end)), expressed in an E. coliexpression system. GenBank Accession Nos. areNM_033445, NM_003528, NM_003532, andNM_003548, respectively. Each histone proteinhas an N-terminal His-tag. Total histone MW =113.8 kDa.
Format: Aqueous buffer solution
Formulation: 20 mM Tris pH 7.5, 1 mMEDTA, 1 mM DTT and 20% glycerol
Genbank: NM_033445, NM_003528, NM_003532, and NM_003548
Host Cell Line: E. coli
Storage Stability: At least 6 months at -80°C.
Uniprot: Q7L7L0, Q16778, P68431, P62805
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. Meagher, R.B., Mussar, K.J., Epigenetics Chromatin 2012 Jul 20;5(1):11.
2. Huang, H., et al., Biosystems 2012 Aug;109(2):214-9.
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