Application Note: For easiest restoration, it is recommended to restore with 1000 µl of 10 mM HCL for a stock concentration of 0.1 mg/ml. This solution can be further diluted in deionized water or other buffers as required. Interleukin-6 (IL-6) protein has been tested by SDS-PGAE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-6 (IL-6) in immunological assays.
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data: Endotoxin Level as determined by kinetic LAL analysis: <0.7 EUs/µg proteinThe activity is determined by the dose-dependent stimulation of mouse 7TD1 cells and is typically less than 1 ng/mL. There is currently no data available for this lot.
Physical State: Lyophilized
Purity and Specificity: Purity greater than 95% as determined by analysis of RP-HPLC and reducing and non-reducing SDS-PAGE against a known standard.
Background: Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contains four conserved cysteine residues involved in two disulfide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulfide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist: the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha-helix is important to the biological activity of the molecule. Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Recombinant Human IL-6 produced in E.coli is a single, non-glycosylated polypeptide chain containing 184 amino acids and having a molecular mass of 21,000 Daltons.
Low Endotoxin: Yes
Other: User Optimized
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