Application Note: TRAIL Recombinant Protein ha been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-TRAIL in immunological assays. Product is provided as lyophilized powder in 0.05M Sodium Chloride and 0.01M Sodium Phosphate, pH 7.5.
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data: Endotoxin Level: Measured by kinetic LAL analysis and is typically ≤ 1 EU/μg protein. Biologic Activity: The activity is determined by the ability to induce apoptotic cell death in TRAIL-sensitive RPMI-8226 cells and is typically 10-15 ng/mL.
Physical State: Lyophilized
Purity and Specificity: TRAIL purity was determined to be greater than 90% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
Background: TNF-Related Apoptosis-Inducing Ligand (TRAIL) is produced by a wide variety of cell types and is shown to be a cytotoxic protein that induces apoptosis in tumor cells through activation of the death receptors, DR4 and DR5. Human TRAIL is active on mouse cells. Recombinant human TRAIL is a non-glycosylated protein, containing the 168 amino acid extracellular portion of TRAIL that is homologous to TNF. It has a molecular weight of 19.5 kDa.
Low Endotoxin: Yes
Other: User Optimized
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