Application Note: TNFα protein has been tested by SDDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-TNFα in immunological assays.
Concentration Value: 0.1 mg/mL
General Disclaimer Note: This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
Other Performance Data: Biological Activity: Recombinant Mouse TNFα is fully biologically active when compared to standard. The ED50 as determined by the cytolysis of mouse L929 cells in the presence of Actinomycin D is 13-20 pg/mL.Endotoxin Level: Measured by LAL is 0.04 EUs/µg (with 50% confidence range).
Physical State: Lyophilized
Purity and Specificity: Purity was determined to be greater than 98% by analysis by RP-HPLC and by reducing and non-reducing SDS- PAGE.
Background: Tumor Necrosis Factor-alpha (TNF-a) is pleiotrophic cytokine secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following stimulation by bacterial LPS . TNF-a activity is mediated via interactions with TNFR1 &TNFR2 receptors. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL-2 & GM-CSF. Recombinant mouse TNF-a produced in E.Coli is a non-glycosylated polypeptide chain containing 156 amino acids and having a molecular mass of 17,300 daltons.
Low Endotoxin: Yes
Other: User Optimized
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