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Encompassing Amino Acids: 801-1147
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: 20 mM HEPES, pH 7.2,2 mM EDTA, 1 mM DTT, and 50 mMpNPP. Reaction was done at RT for 5 min,and monitored at Abs405 nm continuously.(Path length is estimated to 0.5 cm) ofpNPP = 18,000 M-1cm-1
Background: PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. The extracellular region of this PTP contains a carbonic anhydrase-like (CAH) domain, which is also found in the extracellular region of PTPRBETA/ZETA. This gene is located in a chromosomal region that is frequently deleted in renal cell carcinoma and lung carcinoma, thus is thought to be a candidate tumor suppressor gene.
Description: Protein tyrosine phosphatase PTPRG, also known as PTPγ (gamma) (GenBank accession No. NM_002841), a.a. 801-1147 (catalytic domain) with N-terminal GST-tag, MW=65.6 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione
Genbank: NM_002841
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: P23470
Unit Definition: One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. C. Van Niekerk and L. Poels Cancer Lett. 1999, 137: 61.
2. S. Liu, et al., Anticancer Res. 2002, 22:3917.
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