Application Note: Activity was measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2 μg/ml (100 μl/well ) can bind Recombinant nCoV-S1. The EC₅₀ of SARS-CoV-2 (COVID-19) Spike S1 protein is 0.25-0.45 μg/ml.
Form: Lyophilized powder
Buffer (with preservative): Reconstitute with distilled water to 0.1-0.5mg/ml. Lyophilized from 0.22μm-filtered PBS (pH7.4), no preservative.
Concentration:
Region Sequence: Val16-Arg685 of SARS-CoV-2 (COVID-19) Spike S1 protein (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.