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Applications: Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications..
Background: SetDB1 (Histone-lysine N-methyltransferase SETDB1) is a methyltransferase that reversibly catalyzes the di and trimethylation of histone 3 (H3) resulting in inhibition of gene transcription within the euchromatic regions of chromosomes. Studies linking the activity of SetDB1 and H3 methylation can elucidate the mechanisms of tumorigenesis in various types of cancers.
Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
Description: The SetDB1 Chemiluminescent Assay Kit is designed to measure SetDB1 activity for screening and profiling applications. The advantage of the SetDB1 Chemiluminescent Assay Kit is a highly specific antibody that recognizes the methylated K9 residue of Histone H3. With this kit, only three simple steps are required. First, S-adenosylmethionine is incubated with a sample containing the assay buffer and the methyltransferase enzyme. Next, the primary antibody is added. Finally, the wells are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
Storage Stability: This assay kit will perform optimally for up to 6 months from date of receipt when materials are stored as directed.
Supplied As: The SetDB1 Chemiluminescent Assay Kit comes in a convenient 96-well or 384-well format, precoated with histone H3 peptide substrate, an antibody against the methylated lysine 9 residue of Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SetDB1 enzyme for 96 or 384 enzyme reactions.
Uniprot: Q15047
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: Dillon SC, Zhang X, Trievel RC, Cheng X. Genome Biology 2005; 6:227.
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