Background: One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic-dependent kinase cdc2, which regulates entry in to mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of cdc2. It is hypothesized that chk 1 induces serine 216 phosphorylation of Cdc 25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating cdc2.
Positive Control: Recombinant proteins conjugated to OVA, GST, His tags and other. Native chk 1 kinase.
Purification Method: Ammonium Sulfate Precipitation.
Source: Sheep were immunized with a protein corresponding to amino acid sequence 1-474 of human chk-1, having a molecular weight of 54 kDa.
References: 1. Wang, Y., et al. 'CHK1 kinase activity assay.' Methods Mol Biol 2004, 281, 143-151.
2. Yarden, R.I., et al. 'BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage.' Nat Genet 2002, 30, 285-289
3. Nakajo, N., et al. 'Involvement of Chk1 kinase in prophase I arrest of Xenopus oocytes.' Dev Biol 1999, 207, 432-444.
UniProt: O14757.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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