Background: The immunogen used for the anti-human ephB1 (Elk receptor) -EXT region was a GST fusion protein ephB1 (Elk receptor) corresponding to amino acid sequence 16-351 of the N terminal, extracellular region, and having a molecular weight of 116 kD. ephB1, previously known as Elk(eph-like kinase), is a receptor tyrosine kinase of the highly tissue restricted family of eph proteins. EphB1 and other ephB family members are type 1 membrane spanning proteins, comprised of immunoglobulin, fibronectin type III, and cysteine rich subdomains in the ecto domain, and the single uninterrupted cytoplasmic tyrosine kinase domain upstream of a carboxyterminal sterile alpha motif (SAM) domain. ephB family proteins bind ephrins of the B class, ligands that are also transmembrane spanning proteins capable of transmitting signals. ephB1 is expressed predominantly in developing neural structures in embryos, and in vascular epithelium of kidney, and other tissues. Upon binding to alternatively oligomerized ephrin B1, ephB1 signals regulation of cell attachment and cellcell assembly. Members of this protein family are implicated in neuronal and vascular cell targeting.
Positive Control: Human brain.
Immunogen: A GST fusion protein ephB1 (Elk receptor) corresponding to amino acid sequence 16-351 of the N terminal, extracellular region, and having a molecular weight of 116 kD.
Purification Method: Ammonium Sulfate Precipitation.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Stein, E., et al. 'Nck recruitment to Eph receptor, EphB1/ELK, couples ligand activation to c-Jun kinase.' J. Biol. Chem., 273, 1303-1308 (1998)
2. Han, D.C., et al. 'EphB1 associates with Grb7 and regulates cell migration.' J. Biol. Chem., 277, 45655-45661 (2002)
3. Vindis, C., et al. 'EphB1 recruits c-Src and p52Shc to activate MAPK/ERK and promote chemotaxis.' J. Cell Biol., 162, 661-671 (2003).
UniProt: P54762 (Human).
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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