Background: Bax is encoded by a 1.5 kb RNA species created by the retention of intron 5 in the bax mRNA transcript. The exon4/intron5 splice junction is evolutionarily conserved and is found in both the human and mouse bax coding regions. The 630 bp intron 5 contributes 60 amino acids to the Bax protein before a stop codon is encountered during translation. The Bax protein is 24 kDa in size and although it contains the BH1, BH2, and BH3 domains identical to Bax, it has a unique carboxyl terminus and does not have a hydrophobic transmembrane domain (Oltvai et al., 1993). Bax mRNA has been found in many mouse tissues using RT-PCR. It is found co-expressed with Bax in lung, stomach, kidney, thymus, lymph node, bone marrow, pancreas, and spleen. Bax mRNA was very abundant in brain and duodenum with almost no Bax mRNA detected in these tissues (Oltvai et al., 1993).
Immunogen: Synthetic peptide corresponding to amino acids 172 to 199 of theBax protein.
Purification Method: Ammonium Sulfate Precipitation.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide.
References: 1. Oltvai, Z.N., et al. Bcl-2 Heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993, 74, 609-619.
UniProt: Q07812.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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