Background: Bub1, a mitotic checkpoint gene first identified in yeast, ensures proper chromosome segregation by delaying anaphase until chromosomes are properly aligned on the mitotic spindle. Mutations in the mitotic spindle assembly checkpoint genes, like yeast Bub1, cause cells to exit mitosis without proper chromosome attachment/alignment, resulting in aneuploidy. Aneuploidy is a form of genetic instability involved in cancer. Recently, a novel homologue of the murine Bub1 gene has been identified and named Bub1b. The yeast mitotic checkpoint pathway is unresolved, but the mammalian pathway remains even less clear. It is important to investigate the role of the Bub1 genes in the mammalian checkpoint as they have been implicated in carcinogenesis, namely colon carcinomas. The aneuploidy and chromosome instability characterizing some tumors is thought to be caused by mutations in Bub1.
Positive Control: Mitotic NIH/3T3 cells
Purification Method: Ammonium Sulfate Precipitation
Concentration: See vial for concentration
Source: Sheep were immunized with recombinant protein corresponding to amino acids 232-603 of the mouse Bub1b protein.
Formulation: Provided as solution in phosphate buffered saline with 0.08% sodium azide
References: 1. Myrie, K.A., et al. Mutation and expression analysis of human Bub1 and Bub1b in aneuploid breast cancer cell lines. Cancer Lett. 2000, 152, 193-199.2. Davenport, J.W., et al. The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner. Genomics 1999, 55, 113-117.
UniProt: Q9Z1S0
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
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