Clone: Polyclonal
Background: The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1a, including Balb/C, CBA, AKR and NZB, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1a). No precipitation reaction is obtained with purified IgG2a(1b) (C57Bl and SJL/J), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2a(1a) in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgG2a(1a) from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. For such use labelled an unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity an performance requirements are available.
Source: Highly purified normal homogenous IgG2a(1a) isolated from mouse serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
Specificity: Precipitating polyclonal Sheep antiSerum to Mouse IgG2a(1a), allotype specific.
Formulation: Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added.
Caution: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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