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Encompassing Amino Acids: 1-577
Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
Assay Conditions: 50 mM HEPES, pH 7.4, 2 mM EDTA, 3 mM DTT, 100 mM NaCl, 50 mM pNPP.
Background: PTPN6 gene is preferentially expressed in a variety of hematopoietic cells, and is an early response gene in lymphokine stimulated cells. The noncatalytic N-terminus of this PTP can interact with MAP kinases and negatively regulates ERK2 and p38 MAP-kinases activity. The PTPN6 was shown to be involved in the regulation of T cell antigen receptor (TCR) signaling, which was thought to function through dephosphorylating the molecules related to MAP kinase pathway.
Description: Protein tyrosine phosphatase SHP-1, also known as PTPN6 (GenBank accession number NM_080549), full length, with N-terminal GST-tag, MW=95 kDa, expressed in an E. coli expression system.
Format: Aqueous buffer solution
Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
Genbank: NM_080549
Storage Stability: >12 months at -80°C
Tags: N-terminal GST-tag
Uniprot: P25440
Unit Definition: One unit will hydrolyze 1 pmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
Warnings: Avoid freeze/thaw cycles.
Biosafety Level: Not applicable (BSL-1)
References: 1. N.E. Kilgore et al., J. Immunol. 2003, 170:4891-4895.
2. J.V. Ravetch & L.L. Lanier Science 2000, 290: 84-9.
3. C. Duchesne et al. J. Biol. Chem 2003, 278:14274-14283.